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The M21 bus route constitutes a public transit line in Manhattan, New York City. The M21 operates between the West Village and the Lower East Side, serving as a Houston Street crosstown. The M9 and M21 are operated by the New York City Transit Authority, and based out of the Michael J. Quill Depot.
Formerly a streetcar line operated by the Third Avenue Railway, it is now the M104 bus route operated by the New York City Transit Authority, a subsidiary of the Metropolitan Transportation Authority. This bus route no longer runs along the entire route of the former streetcar.